Insulin from bovine pancreas

powder, BioReagent, suitable for cell culture

 

CAS Number                                                          11070-73-8

Formula                                                                  C₂₅₄H₃₇₇N₆₅O₇₅S₆

Formula Weight                                                     5,733.49 g/mol

Suitability                                                                suitable for cell culture

Potency (HPLC)                                                     ≥ 25 USP units/mg dry basis

Zinc (dry basis)                                                      ≤ 1.0 %

Appearance (Color)                                              White to Off White

Appearance (Form)                                               Powder

Solubility (Color)                                                    Colorless

Solubility (Turbidity)

(2 mg/mL in H₂O adjusted to pH 2.0 with HCl)   Clear

Loss on Drying                                                       ≤ 10 %

Nitrogen (Dry Basis)                                              14.5 - 16.5 %

Cell Culture Test                                                    Pass

Recommended Retest Period                            2 years

Storage Temperature                                          -20 °C

Gene Information                                                  cow ... INS(28082

                                                                                     

Biochem/physiol Actions: Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat.

Reconstitution: Insulin has low solubility at neutral pH. It can be solubilized at 2 mg/ml in dilute acetic or hydrochloric acid, pH 2-3.

Physical form:      powder: -0 °C;      solution stable: 2-8 °C, 1 year

Application:

Insulin is used as a growth factor in many mammalian cell culture systems.
• Insulin from bovine pancreas has been used for the differentiation of MSCs (mesenchymal stem cells) to adipocytes. 
• It has been used for culturing of various cells. 
• It has also been used to study insulin-mediated inhibition of the HMGCS2 (3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase) gene.



(Bayville Chemical Supply only offers this chemical for research and laboratory use. We do not supply this in bulk or for human.)